Testimonials

Mesenchymal stem cells

"GeneCellin is fantastic. I recommended it to some of my colleagues here in York, and they have managed to transfect primary human mesenchymal stem cells with high efficiency, whereas previously they have never been able to transfect these cells and they have been trying for years. ..." - Dr. P. Prior, UK

Primary myoblast cells

" Actually, we use it routinely to transfect phosphorothioate oligos into cultured myoblasts in the context of a gene therapy project. Genecellin was one of the most efficient reagents in our tests for transfection efficiency and was never toxic, even at higher doses...." - Dr. R. Pelletier, Canada

GeneCellin Transfection Reagent

The power of a polymer

GeneCellin^TM is a powerful polymer-based in vitro transfection reagent which enables the achievement of higher efficiencies in the delivery of plasmid DNA into cell lines and primary cells compared to other reagents.

This innovative transfection reagent is a stable formulation of nanoparticules called “nanoporters”. This technology allows to achieve superior and reproducible transgene expression levels without any toxic effect.

Advantages

Very simple to use : Quick one-tube protocol

High efficiency & reproducibility

Maximum cell viability

Low amount of DNA required

Serum compatible

Very low toxicity

Applications

Stable & transient transfections

DNA Co-transfections

Reverse transfections

Neuroblastoma cells focus

Skin cancer focus

HeLa Cell

Hela cell transfection

Hepitelial Cell

Epithelial cells transfection

HeLa cells were transfected with 0.25 µg of plasmid DNA per well in a 24-well plate.

Result : arround 99 % transfected with a perfect viability

Epithelial cells were transfected with 0.25 µg of plasmid DNA per well in a 24-well plate.

Result : 85 % transfected with a perfect viability

GeneCellin and other reagents

HeLa cells were transfected with 0.25 µg of plasmid DNA per well in a 24-well plate and several commercial transfection reagents according to the manufacturer's protocols.

Transfection reagent comparison

GeneCellin requires a small amount of plasmid DNA. Using less DNA reduces adverse cytotoxic effects triggered by transfection and maintains highcell viabilitywith high transfection efficiency.

GFP expression in various cell lines transfected with 0.5 µg of plasmid DNA and 2 µL of GeneCellin^TMTransfection Reagent in a 24-well plate.

PUBLICATION LIST
Date	Cell	Product	Who	Paper	Author	Page
2010	Cos-1, HEK	GeneCellin	St. Jude Children’s Research Hospital, Memphis, USA	Molecular Cell	Shimizu	924
2011	HEK	GeneCellin	Institut de Investigació Biomèdica de Girona, Espagne	Journal of Proteome Research	Beltran-Alvarez	5
2011	BE(2)-M17, HEK, N2a, U87	GeneCellin	Université de Sherbrooke, Sherbrooke, Quebec, Canada	The FASEB Journal	Vanderperre	3
2012	Cos-1, MDCK, U2OS	GeneCellin	Albert Einstein College of Medicine, Bronx, USA	The Journal of Biological Chemistry	Lai	2
2012	Cos-1, HEK	GeneCellin	St. Jude Children’s Research Hospital, Memphis, USA	The Journal of Biological Chemistry	Howes	3
2012	NRK	GeneCellin	University of York, UK	Methods in Enzymology	Pryor	150 151
2012	HEK	GeneCellin	Semmelweis University, Budapest, Hongrie	Journal of Cell Science	Toth	15
2012	GM-7373	GeneCellin	Julius-Bernstein-Institut, Universitat Halle-Wittenberg, Halle, Allemagne	Free Radical Biology & Medicine	Ruhs	5
2012	MEF	GeneCellin	St. Jude Children’s Research Hospital, Memphis, USA	Plos One	Mann	9
2012	HEK	GeneCellin	Université de Sherbrooke, Sherbrooke, Quebec, Canada	The Journal of Neuroscience	Béland	13256
2012	Cos	GeneCellin	National Institute for Medical Research, London, UK	Scientific Reports	Menon	6
2012	HEK, HeLa	GeneCellin	University of York, UK	The Journal of Biological Chemistry	Miller	3

Ordering information
Reference	Size	Number of transfection in a 24-well plate	Prices	Sippment	Storage
GC500	500 µL	250	Price list	Room temperature	1 year at 4°c
GC1000	1000 µL	500
GC5000	5 x 1000 µL	2500

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