All kits contain a fluorescent control antibody

ImmunoCellin-Nw Antibody Delivery Reagent

The New and Innovative Tool for your Research

A unique reagent  for antibody intracellular delivery

BCC introduce a very efficient antibody intracellular delivery reagent based on a liposomal formulation.

Unique, very easy to use, ImmunoCellin deliver any antibody into live cells in few hours only.

Advantages

Very easy to use

BSA compatible

Efficient with any antibody

Serum compatible

It enables the use of thousands of existing antibodies as drug discovery tools or as new drug entities. The delivered antibodies retain their structure and function since no organic chemical coupling occurs. Furthermore, this system is not inhibited by the presence of serum or BSA, which makes its use possible for in vivo approaches.

New fields of investigation

Antibodies are not able to pass through plasmic membranes of live cells towards potential intracellular targets. Thousands of antibodies have been developped as tools for the scientists. Their intracellular delivery opens new fields of investigation for both research and therapeutic applications.

This system is very well adapated to modulate intracellular protein functions. For example, siRNA intracellular delivery allows to shut down the expression and all the functions of the targeted protein. However, if this protein has several different function inside cells, it is more advisable to inhibit only one of them by using a specific monoclonal antibody.

    Prevent protein-protein interactions

    Disrupt molecular mechanisms

    Identify targets

    Identify side effects of your molecule

ImmunoCellin functions by encapsulating the antibodies through non-covalent interactions. Since no organic chemical coupling occurs, the delivered antibodies retain their structure and function. This reagent is efficient even in the presence of serum.

Non-specific antibody

0.25 µg of a DyLight labeled Mouse non-specific antibody has been delivered into Human NIH3T3 cells in a 96-well plate with 0.5 µL of ImmunoCellin reagent.

Result: The antibody localizes non specifically into the cytosol

Anti-Nucleoporin p62 antibody

0.25 µg of a FITC Mouse Anti-Nucleoporin p62 antibody (BD Transduction Laboratories) has been delivered into Human Lung cells in a 96-well plate with 0.5 µL of ImmunoCellin reagent.

Result: The antibody accumulates onto the nuclear envelope